BAT-NPS are used for the detection and determination of colony count of Alicyclobacillus sp. in sugar, syrup, fruit juices and other beverages. Bacteria of genus Alicyclobacillus have optimal growth conditions at a low pH value and an increased incubation temperature. Their development is enhanced by the composition of the BAT-NPS. The growth of accompanying microorganisms is inhibited by the low pH value and high incubation temperature. The medium is manufactured and quality tested in compliance with ISO 11133:2014 standard.